Chicopros - Intellectual virtual library

August 29, 2013 – 01:53
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chicopros - Intellectual virtual library science - textbooks

    Microsatellite DNA markers by Francisco Prosdocimi The microsatellite DNA consists of units of about from 1 to 6 nucleotides repeated in tandem (in series) within the genome of various organisms, eukaryotic pricipalmente.

    The microsatellites are used as molecular markers due to the high level of polymorphism found in their locos, which provides their use in various purposes of the study population, allowing the analysis from individuals to closely related species.

    The slipping (slippage) DNA polymerase during DNA replication is regarded as the main cause of variation in number of repeats these loci. The different numbers of repeats characterize the different alleles can be detected in polyacrylamide gel electrophoresis.

    As the microsatellite DNA typically does not carry information, additions or deletions of repeating units are not selected, providing high variability of alleles.

    History

    The satellite DNA was discovered in 1960. Scientists have found that when centrifugavam DNA under certain conditions, this patient was in two or more layers: one main band containing the genes, and secondary bands, which bands have been called satellites. The satellite bands showed up as being composed of repeated DNA sequences and very long.

    In 1985, Alec. J. Jeffreys, University of Leicester found smaller regions containing repetitive DNA sequences, which called minisatellites, which consisted of 15 repetitions or more base pairs. Jeffreys and his colleagues also determined that the number of repetitions of a given minisatélite differed between individuals, a procedure that led to the subsequent invention of the technique of DNA fingerprinting.

    In the late 80's James L. Weber and Paula L. May, the Marshfield Medical Research Foundation, and Micahel Jeffrey Litt and A. Luty, Oregon Health Sciences University isolated compounds satellite repeats of bases even smaller and called these microsatellite repeats.

    Microsatellites as agents of Evolution

    An important role in the evolution of microsatellites have been shown in pathogenic bacteria such as Neisseria gonorrhoeae, Haemophilus influenzae, Helicobacter pylori, Mycoplasma spp. and Streptococcus spp. In all the described organisms, changes in the number of microsatellite repeats cause the production of proteins that differ slightly in certain environmental conditions, are most useful for the pathogen of the protein with the standard number of repetitions, providing a better adaptation to the environment (van Belkun, 1999).

Source: www2.bioqmed.ufrj.br

National Academies Press Medically Assisted Conception: An Agenda for Research
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Seems to me the website is just

2008-11-04 15:47:45 by Kristy2078

An informational website with input from and maintained by the Oregon Fibromyalgia Foundation, compromised of medical personnel (i.e. doctors, researchers) at OHSU.
I don't see any sign-up anywhere. I did find a page where someone can sign up to be a research volunteer. I am guessing that's where aspianato signed up and got the information about the conference.

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